Journal: International Journal of Molecular Sciences

Article Title: A New Hypoglycemic Prenylated Indole Alkaloid N -Oxide from Endophytic Fungus Pallidocercospora crystalline

doi: 10.3390/ijms24108767

Figure Lengend Snippet: Compounds increased glucose uptake without any apparent toxicity: ( A ) The β-cell number of each compound-treated double transgenic zebrafish Tg(−1.2ins:htBid TE-ON ; LR) Tg(−1.2ins:H2Bmcherry) . Total of 10 μM of each compound was added at 5 dpf after the β-cell ablation for 24 h. ( B ) The glucose uptake indicated by 2-NBDG fluorescence. The larvae of each group were induced with doxycycline and tebufenozide except the group of tBid in 3 dpf for 48 h. Total of 10 μM of each compound was added at 5 dpf after the β-cell ablation for 24 h. Then, 2-NBDG was incubated for 3 h in dark. RGZ: rosiglitazone. The values shown are means ± SEM from three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 via one-way ANOVA. ( C ) Morphology of 24 hpf embryos or 72 hpf zebrafish larvae treated with compounds. ( D ) Blood vessel morphology of 6 dpf zebrafish larvae Tg(flil:eGFP) treated with compounds.

Article Snippet: After the compound treatment, zebrafish (6 dpf) were incubated in a culture medium containing 600 μM 2-NBDG (Apexbio, B6035, Houston, TX, USA) for 3 h. A pool of 5 larvae was homogenized in 100 μL of sample buffer.

Techniques: Transgenic Assay, Fluorescence, Incubation